Download Lab protocols used in the AFLP analysis and for preparation of samples for automated sequencing.
Lab Sheets
Extract DNA Amplify DNA by PCR Restrict PCR-Products
Prepare Agarose-Gel Run Electrophoresis Use Micropipettors
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Workflow
1 Prepare biological sample, e.g. cheek cells
2 Extract mitochondrial DNA
3 Perform PCR for clade specific region of most abundant haplotype
4 Prepare agarosegel
5 Test for successful PCR
6 Perform REnzyme action on PCR products
7 Evaluate complete REnzyme digest and analyse digest fragments
8 Assign haplotype
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