1 Prepare biological sample, e.g. cheek cells
2 Extract mitochondrial DNA
3 Perform PCR for clade specific region of most abundant haplotype
4 Prepare agarosegel
5 Test for successful PCR
6 Perform REnzyme action on PCR products
7 Evaluate complete REnzyme digest and analyse digest fragments
8 Assign haplotype
or repeat from step 3 with next most abundant haplotype
alternative workflow:
1 ...
2 ...
3 Perform PCR for HVR1 a/o HVR2- region
4 ...
5 ...
6 Estimate DNA-concentration
7 Submit to sequencing
8 Assign haplotype